| 1059 ANAEROCELLUM MEDIUM
|
|
|
| 酵母提取物 (Difco) |
0.3 |
g
|
| Na2-succinate |
1.0 |
g
|
| (NH4)-acetate |
0.5 |
g
|
| Fe(III) citrate solution (0.1% in H2O) |
5.0 |
ml
|
| KH2PO4 |
0.5 |
g
|
| MgSO4 x 7 H2O |
0.4 |
g
|
| NaCl |
0.4 |
g
|
| NH4Cl |
0.4 |
g
|
| CaCl2·2 H2O |
0.05 |
g
|
| Vitamin B12 solution (10 mg in 100 ml H2O) |
0.4 |
mlg
|
| Trace element solution SL-10（见培养基1064） |
1.0 |
ml
|
| L-Cysteiniumchloride |
0.3 |
g
|
| Resazurin(0 |
|
|
| 1%) |
0.5 |
ml
|
| 蒸馏水 |
1.0 |
L
|
| Adjust pH to 6.8 |
|
|
| Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
|
|
|
