| 0989
|
|
|
| KH2PO4 |
0.3 |
g
|
| K2HPO4 |
0.3 |
g
|
| (NH4)2SO4 |
0.3 |
g
|
| NaCl |
0.6 |
g
|
| MgSO4·7H2O |
0.13 |
g
|
| FeSO4·7H2O |
2.0 |
mg
|
| CaCl2·2H2O |
8.0 |
mg
|
| Yeast extract (Difco) |
2.0 |
g
|
| Trypticase peptone (BBL) |
2.0 |
g
|
| Sodium acetate |
2.5 |
g
|
| Sodium formate |
2.5 |
g
|
| Trace minerals (见培养基0893) |
10.0 |
ml
|
| Trace vitamins (见培养基0901) |
10.0 |
ml
|
| NaHCO3 |
5.0 |
g
|
| Na2S·9H2O |
0.5 |
g
|
| L-Cysteine·HCl·H2O |
0.5 |
g
|
| Resazurin |
1.0 |
mg
|
| Distilled water |
1.0 |
L
|
| Mix ingredients |
|
|
| except NaHCO3 |
|
|
| L-Cysteine·HCl·H2O and Na2S·9H2O |
|
|
| and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20) |
|
|
| and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g. |
|
|
| 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine·HCl and Na2S·9H2O as 5% solutions |
|
|
| respectively |
|
|
| under a N2 atmosphere. Prior to inoculation |
|
|
| aseptically and anaerobically add the cysteine·HCl and Na2S·9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).
|
|
|
